Gramicidin Bioactivity Database (GBD) is a resource platform which contains various information about anticancer and antimicrobial activities of gramicidin. Gramicidin is antibiotic produced by Bacillus brevis isolated from soil samples. It was the first antibiotic used in clinical treatments in 1939 and facilitated the subsequent application of other antibiotics such as penicillin. In nature, gramicidin is usually a mixture of linear peptide gramicidin A (about 85%) or cyclic peptide gramicidin S. The gramicidin A acts as an ion channel in the form of a dimer which causes the efflux of intracellular monovalent cations, while the gramicidin S can cause membrane lysis. This database contains 2,679 pieces of information about the anticancer and antimicrobial activities, chemical structures, targets, mechanisms of action of gramicidin and their analogues, and types and strains of corresponding cancer cells/bacteria are also included. This database aims to help researchers in quickly grasping the activity information of gramicidin and referring to the influence of modifications in different analogues on the overall activity, thereby providing inspiration for the further modifications in gramicidin. Gramicidin as antibiotic applied in last century, has been used in the field of antimicrobial treatment for a long time, However, its potential values especially in the field of cancer treatment are needed to be further investigated. As this database continues to be updated, it will broaden the application of gramicidin in clinical treatments and promote the research of peptide drugs.
